Topical Update

Volume 2, Issue 2, July 2007

Dr. CHEUK Wah

BSc(Hons), MBBS, FHKCPath, FRCPA, Associate Consultant, Department of Pathology, Queen Elizabeth Hospital 

Overview of conventional molecular techniques in lymphomas

The use of molecular techniques in hematolymphoid pathology started with cloning of the immunoglobulin and T cell receptor genes. [1] This is followed by the cloning of a number of translocation breakpoints in some common lymphoma types.[2-4] Assay of chromosomal breakpoints not only helps in confirming a clonal proliferation but also prov ides an indication of the type of lymphoma. The main application is to establish clonality or lineage of a lymphoid proliferation. 

Southern blot...

Volume 2, Issue 1, March 2007

Dr Edmond S K Ma

MD (HK), FRCPath, FHKAM (Pathology)

Department of Pathology, Hong Kong Sanatorium & Hospital

Background

G6PD catalyzes the conversion of glucose-6-phosphate (G6P) to 6-phosphogluconate concurrent with reduction of NADP to NADPH, which in turn acts through glutathione and catalase pathways to detoxify hydrogen peroxide, thus counteracting oxidative stress to the cell. In the body, red cells are most susceptible to oxidative damage because oxygen radicals are generated continuously as haemoglobin cycles from deoxygenated to oxygenated forms, as well as being readily exposed to exogenous oxidizing agents present in the blood. Hence G6PD deficiency is a prototype cause of haemolytic anaemia due to intrinsic red cell enzyme...

Volume 1, Issue 3, November 2006

Dr Ching-Wan LAM, MBChB(CUHK), PhD(CUHK), FRCPA, FHKAM(Pathology)

Associate Professor, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital

Dr Chloe MAK, MBBS(HK), FRCPA

Resident Specialist, Division of Clinical Biochemistry, Department of Pathology, Queen Mary Hospital

Wilson disease (WD) (MIM # 277900) is an autosomal recessive disorder of copper transport. Clinical manifestations of WD vary widely. The age of onset ranges from three to more than 50 years of age. The initial onset of symptoms can be hepatic, neurological, psychiatric or as an acute haemolytic crisis. The prevalence of WD has been estimated to be approximately 1 in 30,000 in the Caucasian population. Although the prevalence of WD in the Hong Kong...

Volume 1, Issue 2, July 2006

Raymond WH Yung

Infection Control Branch, Centre for Health Protection, Department of Health & Infectious Disease Control Training Centre, Hospital Authority

In the past three years, we have witnessed the revived recognition of the importance of the specialty of Clinical Microbiology and Infection. The SARS outbreak reminded the medical profession that the line of defence which we had built against infection was still not robust enough to handle major outbreaks. Three reports were published after the outbreak. They outlined the deficiencies found and recommended what should be done for the future.1-3 Many of the recommendations are relevant and will impact on the future development of the specialty of Clinical Microbiology and Infection. Let me quote from the report of the...

Volume 1, Issue 1, March 2006

JSM Peiris & Wilina Lim

Department of Microbiology, The University of Hong Kong & Virology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR 

Avian influenza A subtype H5N1 is endemic in poultry across south-east Asia and continues to cause zoonotic disease in humans. So far, transmission of virus from avian to humans appears very inefficient and sustained transmission from human-to-human has not occurred. However, with the continued opportunity for human exposure over an ever increasing geographic range, it is possible (though not inevitable) that H5N1 virus may acquire the ability to transmit efficiently from human-to-human, leading to a pandemic.

Human disease caused by H5N1 influenza...